ABSTRACT
Objective: To determine the susceptibility and resistance pattern of bacteria and fungi isolates obtained from herbal anti-infective liquid preparations manufactured and marketed in South-East Nigeria to conventional antibiotics. Study Design: Experimental Place and Duration of the study: Pharmaceutical Microbiology and biotechnology Laboratory, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Agulu Campus between October 2011 and March 2012. Methodology: Isolation and characterization of contaminating microorganisms were carried out using standard procedures. A total of forty-nine (49) bacteria and forty (40) fungi isolated from the herbal products were examined for susceptibility to conventional antibiotics using the disc diffusion method. The bacterial isolates were tested against ciprofloxacin, ofloxacin, amoxicillin-clavulanic acid, gentamicin, cefotaxime, ceftazidime, ceftriazone, sulphamethoxazole, tetracycline and ampicillin were employed while fungi isolates were tested against five common antifungal-griseofulvin, nystatin, ketoconazole, fluconazole and clotrimazole. The Multiple Antibiotic Resistance Index (MARI) of each of the isolated bacteria was obtained following the standard method. Result: The antimicrobial susceptibility-resistance profile of the bacteria isolates revealed that most of the bacteria were sensitive to ciprofloxacin, ofloxacin, gentamicin, and ceftriaxone, On the other hand, a good number of the isolates demonstrated high level of resistance to common antibiotics like Ampicillin, amoxycillin-clavulanic acid, trimethoprimsulphamethoxazole, and moderate level of resistance to Tetracycline, and some of the third generation cephalosporins - ceftazidime and cefotazime. Multiple Antibiotic Resistance Index (MARI) evaluation revealed that most of the isolates were resistance to more than fifty percent (50%) of the number of antibiotics used. The fungal isolates were susceptible to nystatin, ketoconazole and clotrimazole, resistance to fluconazole and high resistance recorded against griseofulvin. Conclusion: The results of this study revealed that the herbal medications can serve as a trail of spread of antibiotic-resistance genes.
Subject(s)
Bacteria/drug effects , Bacteria/isolation & purification , Drug Resistance, Microbial/epidemiology , Drug Resistance, Microbial/etiology , Fungi/drug effects , Fungi/isolation & purification , Herbal Medicine/economics , Nigeria , Plant Preparations/biosynthesis , Plant Preparations/economics , Plant Preparations/microbiologyABSTRACT
The survival and sustenance of man depends largely on plants which generate directly 87 percent of its food needs and constitute a source of basic health care in developing countries. Based on socio-economic surveys and field observations led in the Rissani oasis (SE of Morocco), we have identified 109 species belonging to 45 botanical families and 102 genera. The distribution in families is: Lamiaceae (15.2 percent, Asteraceae (11.5 percent), Fabaceae (8.46 percent, Poaceae (8.12 percent) and Apiaceae (6.75 percent). The species used in traditional medicine correspond to 57.8 percent, for food 10.1 percent and for other uses 28.4 percent. Of these species, 10.1 percent are cultivated, naturalized, introduced and/or weeds. Byproducts of 46.8 percent of these species are imported from other regions of Morocco and locally marketed. Many medicinal species from this area are not recognized by the inhabitants, and their sensitization towards the use and conservation of local plant diversity is needed.
La supervivencia y sustentabilidad de la humanidad depende en gran medida de las plantas. Estas satisfacen directamente el 87 por ciento de sus necesidades alimenticias y constituyen, en países en desarrollo, una fuente para el cuidado de salud. Basados en estudios y observaciones de campo realizadas en el oasis de Rissani (SE de Marruecos), hemos identificado las 109 especies de plantas más utilizadas que pertenecen a 45 familias y 102 géneros. La distribución por familia es: Lamiaceae (15.2 por ciento), Asteraceae (11.5 por ciento), Fabaceae (8.46 por ciento), Proaceae (8.12 por ciento) y Apiaceae (6.75 por ciento). Las especies utilizadas en medicina tradicional corresponden al 57.8 por ciento, en alimentación 28.5 por ciento y para usos múltiples 28.4 por ciento. De estas especies el 10.1 por ciento son cultivadas, naturalizadas, introducidas y/o corresponden a malezas. Subproductos del 48.8 por ciento de estas especies son importados de otras regiones de Marrueco para su comercialización. Muchas de las especies medicinales no son reconocidas por los habitantes del oasis y es necesario sensibilizarlos en relación a su utilización y conservación.